Publications

Tam J, Icho S, Utama E, Orrell KE, Gómez-Biagi RF, Theriot CM, Kroh HK, Rutherford SA, Lacy DB, Melnyk RA. (2020). Intestinal bile acids directly modulate the structure and function of C. difficile TcdB toxin. PNAS. 117(12):6792-6800.

Lim S, Khoo R, Peh KM, Teo J, Chang SC, Ng S, Beilhartz GL, Melnyk RA, Johannes CW, Brown CJ, Lane DP, Henry B, Partridge AW. (2020). bioPROTACs as versatile modulators of intracellular therapeutic targets: Application to proliferating cell nuclear antigen (PCNA). PNAS. 117(11):5791-5800.

Arnold AE, Smith LJ, Beilhartz GL, Bahlmann LC, Jameson E, Melnyk RA, Shoichet MS. (2020). Attenuated diphtheria toxin mediates siRNA delivery. SCIENCE ADVANCES. 6: 1-13. Accepted

Orrell KE, Mansfield MJ, Doxey AC, Melnyk RA. (2020). The C. difficile toxin B membrane translocation machinery is an evolutionarily conserved protein delivery apparatus. NATURE COMMUNICATIONS. 23(11): 1-11.

Jardine S, Anderson S, Babcok S, Leung G, Pan J, Dhingani N, Warner N, Gui C, Siddiqui I, Kotlarz D, Dowling JJ, Melnyk RA, Snapper SB, Klein C, Thiagarajah JR, Muise AM. (2020). Drug Screen Identifies Leflunomide for Treatment of Inflammatory Bowel Disease Caused by TTC7A Deficiency. GASTROENTEROLOGY. 5085(19): 141574-6.

Icho S, Melnyk RA. (2019). Dismantling a Toxin to Disarm a Superbug. TRENDS IN PHARMACEUTICAL SCIENCES. 19: 1-2.

Lai MT, Tawa P, Auger A, Wang D, Su HP, Yan Y, Hazuda DJ, Miller MD, Asante-Appiah E, Melnyk RA. (2018). Identification of novel bifunctional HIV-1 reverse transcriptase inhibitors. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY. 73: 109-117.

Kroh K, Chandrasekaran R, Zhang Z, Rosenthal K, Woods R, Jin X, Nyborg A, Rainey GJ, Warrener P, Melnyk RA , Spiller B, Lacy DB. (2018). A neutralizing antibody that blocks Clostridium difficile toxin TcdB enzyme delivery. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 293(3): 941-952.

Orrell KE, Tellgren-Roth Å, Di Bernardo M, Zhang Z, Cuviello F, Lundqvist J, von Heijne G, Nilsson I, Melnyk RA. (2018). Direct Detection of Membrane-Inserting Fragments Defines the Translocation Pores of a Family of Pathogenic Toxins. JOURNAL OF MOLECULAR BIOLOGY. 430: 3190-3199.

Park M, Xu X, Min W, Sugiman-Marangos SN, Beilhartz GL, Adams JJ, Sidhu SS, Grunebaum E, Melnyk RA. (2018). Intracellular Delivery of Human Purine Nucleoside Phosphorylase by Engineered Diphtheria Toxin Rescues Function in Target Cells. MOLECULAR PHARMACEUTICS. 15(11): 5217-5226.

Mansfield MJ, Sugiman-Marangos SN, Melnyk RA, Doxey AC. (2018). Identification of a diphtheria toxin-like gene family beyond the Corynebacterium genus. FEBS LETTERS. 592(16): 2693-2705.

Tam J, Hamza T, Ma B, Chen K, Beilhartz GL, Ravel J, Feng H, Melnyk RA. (2018). Host-targeted niclosamide inhibits C. difficile virulence and prevents disease in mice without disrupting the gut microbiota. NATURE COMMUNICATIONS. 7: 1-11.

Orrell KE, Tellgren-Roth Å, Zhang Z, Cuviello F, Di Bernardo M, Lundqvist J, von Heijne G, Nilsson I, Melnyk RA. (2018). Direct Detection of Membrane-Inserting Fragments Defines the Translocation Pores of a Family of Pathogenic Toxins. JOURNAL OF MOLECULAR BIOLOGY. 430(52): 461-473.

Orrell KE, Zhang Z, Sugiman-Marangos SN, Melnyk RA. (2017). Clostridium difficile toxins A and B: Receptors, pores, and translocation into cells.CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY. 52(4): 461-473.

Gupta P, Zhang Z, Sugiman-Marangos SN, Tam J, Raman S, Julien JP, Kroh HK, Lacy DB, Murgolo N, Bekkari K, Therien AH, Hernandez LD, Melnyk RA. (2017). Functional defects in Clostridium difficile TcdB toxin uptake identify CSPG4 receptor binding determinants. JOURNAL OF BIOLOGICAL CHEMISTRY. 292(42): 17290-17301.

Beilhartz GL, Sugiman-Marangos SN, Melnyk RA. (2017). Repurposing bacterial toxins for intracellular delivery of therapeutic proteins. BIOCHEMICAL PHARMACOLOGY. 2952(7): 30198-3. , Open Access?: No

Chumbler NM, Rutherford SA, Zhang Z, Farrow MA, Lisher JP, Farquhar E, Giedroc GP, Spiller BW, Melnyk RA, Lacy DB. (2016). Crystal structure of Clostridium difficile Toxin A. NATURE MICROBIOLOGY. 1(1): 26-36.

Belihartz, GL., Tam, J., Zhang, Z., Melnyk, RA. (2016). Comment on “A small-molecule antivirulence agent for treating Clostridium difficile infection”. SCIENCE TRANSLATIONAL MEDICINE. 21(8): 370-373.

Hamza T, Zhang Z, Melnyk RA, Feng H. (2016). Defective mutations within the translocation domain of Clostridium difficile toxin B impair disease pathogenesis. PATHOGENS AND DISEASE. 74(1): 1-5.

Baker P, Hill PJ, Snarr BD, Alnabelseya N, Pestrak MJ, Lee MJ, Jennings LK, Tam J, Melnyk RA, Parsek MR, Sheppard DC, Wozniak DJ, Howell PL. (2016). Exopolysaccharide biosynthetic glycoside hydrolases can be utilized to disrupt and prevent Pseudomonas aeruginosa biofilms. SCIENCE ADVANCES. 20(5): 1-9.

Auger A, Park M, Nitschke F, Minassian LM, Beilhartz GL, Minassian BA, Melnyk RA. (2015).Efficient Delivery of Structurally Diverse Protein Cargo into Mammalian Cells by a Bacterial Toxin. MOLECULAR PHARMACEUTICS. 12(8): 2962-71. , Open Access?: No

Bernatchez JA, Paul R, Tchesnokov EP, Ngure M, Beilhartz GL, Berghuis AM, Lavoie R, Li L, Auger A, Melnyk RA, Grobler JA, Miller MD, Hazuda DJ, Hecht SM, Götte M. (2015). Derivatives of Mesoxalic Acid Block Translocation of HIV-1 Reverse Transcriptase. JOURNAL OF BIOLOGICAL CHEMISTRY. 290(3): 175-85.

Tam, J., Beilhartz, G.L., Auger, A., Gupta, P., Therien, A.G., Melnyk, R.A. (2015). Small Molecule Inhibitors of Clostridium difficile Toxin B-Induced Cellular Damage. CHEMISTRY & BIOLOGY. 22: 1-11.

Beilhartz GL, Tam J, Melnyk RA. (2015). Small Molecules Take A Big Step Against Clostridium difficile. TRENDS IN MICROBIOLOGY. 23(12): 746-748.

Zhang Z , Park M , Tam J , Auger A , Beilhartz GL , Lacy DB , Melnyk RA. (2014). Translocation domain mutations affecting cellular toxicity identify the Clostridium difficile toxin B pore. PNAS. 111: 3721-6.

Full list can be found here

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